Autoradiographic detections of 111indium-labeled platelets in brain tissue sections.
نویسندگان
چکیده
Various techniques for detection of blood platelets in tissue section were evaluated in an incremental air embolism model of ischemia in order to further investigate the accumulation of platelets in brains subjected to cell-damaging ischemia. The experimental animals were dogs anesthetized with alpha-chloralose. A new autoradiographic technique was devised that allows precise localization of 111Indium-labeled platelets in brain sections. The technique is described in detail along with some effects of the label on platelet function and behavior. The technique can be performed in conjunction with 14C-iodoantipyrine autoradiographic measurement of blood flow without causing mutual interference or other forms of interaction. This permits the simultaneous investigation of local blood flow and deposition of platelets.
منابع مشابه
Autoradiographic observations of the induced vascular injuries by arachidonic acid in rabbit's brain and lung...using 111In-oxine labeled platelets
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عنوان ژورنال:
- Stroke
دوره 15 6 شماره
صفحات -
تاریخ انتشار 1984